aiib2 β1 integrin (Developmental Studies Hybridoma Bank)
Structured Review
Aiib2 β1 Integrin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aiib2+%CE%B21+integrin/anti-Integrin/pm41882003-400-124-127
Average 95 stars, based on 20 article reviews
Images
Related Articles
Blocking Assay:Article Title: Extracellular matrix rigidity controls breast cancer metastasis via TYK2-mediated mechanotransduction. Article Snippet: Antibodies were conjugated to protein G beads (LYN) or protein A beads (TYK2) (Invitrogen), crosslinked using disuccinimidyl suberate (Pierce) as per manufacturer’s protocol, incubated with lysates overnight at 4°C, washed eight times with IP lysis buffer supplemented with 200 mM NaCl, and eluted by incubation in LDS sample buffer with 50 mM DTT at 70°C for 10 minutes. Control:Article Title: Extracellular matrix rigidity controls breast cancer metastasis via TYK2-mediated mechanotransduction. Article Snippet: Antibodies were conjugated to protein G beads (LYN) or protein A beads (TYK2) (Invitrogen), crosslinked using disuccinimidyl suberate (Pierce) as per manufacturer’s protocol, incubated with lysates overnight at 4°C, washed eight times with IP lysis buffer supplemented with 200 mM NaCl, and eluted by incubation in LDS sample buffer with 50 mM DTT at 70°C for 10 minutes. Western Blot:Article Title: Extracellular matrix rigidity controls breast cancer metastasis via TYK2-mediated mechanotransduction. Article Snippet: Antibodies were conjugated to protein G beads (LYN) or protein A beads (TYK2) (Invitrogen), crosslinked using disuccinimidyl suberate (Pierce) as per manufacturer’s protocol, incubated with lysates overnight at 4°C, washed eight times with IP lysis buffer supplemented with 200 mM NaCl, and eluted by incubation in LDS sample buffer with 50 mM DTT at 70°C for 10 minutes. Expressing:Article Title: Extracellular matrix rigidity controls breast cancer metastasis via TYK2-mediated mechanotransduction. Article Snippet: Antibodies were conjugated to protein G beads (LYN) or protein A beads (TYK2) (Invitrogen), crosslinked using disuccinimidyl suberate (Pierce) as per manufacturer’s protocol, incubated with lysates overnight at 4°C, washed eight times with IP lysis buffer supplemented with 200 mM NaCl, and eluted by incubation in LDS sample buffer with 50 mM DTT at 70°C for 10 minutes. Immunoprecipitation:Article Title: Extracellular matrix rigidity controls breast cancer metastasis via TYK2-mediated mechanotransduction. Article Snippet: Antibodies were conjugated to protein G beads (LYN) or protein A beads (TYK2) (Invitrogen), crosslinked using disuccinimidyl suberate (Pierce) as per manufacturer’s protocol, incubated with lysates overnight at 4°C, washed eight times with IP lysis buffer supplemented with 200 mM NaCl, and eluted by incubation in LDS sample buffer with 50 mM DTT at 70°C for 10 minutes. |

